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The purpose of this study was to investigate the use of micropropagation protocols to assist in the
early maturation and potential precocious flowering of Doryanthes excelsa. This has implications for commercial cutflower production as it is reported to take 10 years or longer for this giant monocot to develop its first flower. The effective horticultural development of Doryanthes is therefore dependent upon the derivation of a propagation method which facilitates rapid multiplication and potential early maturation. Key factors investigated in this study include the use of immature inflorescence tissues as explant source material, sterilization and culture conditions, control of phenolic exudates in explant preparation and the selection of appropriate growth regulator levels to achieve in vitro regeneration. Future directions involving the in vitro culture of this giant monocotyledon are discussed.